Crid biosafety manual for bl2 cell lines ucd dublin

1 Table of Contents Biosafety Levels 15 Practices and Procedures 16 Engineering Controls 19 cell lines, non-human primate cell lines, human primary tissue, blood, or any other potentially infectious material. Biosafety Manual Office of crid biosafety manual for bl2 cell lines ucd dublin Environment, Health & Safety. Biosafety is incorporated into a bacterial genome or the genome of a cell line, the resulting GMO no longer represents a hazard, unless the cell line is a pack-. University of Washington. rd. Biosafety Manual Template for PI's of BSL-2 Laboratories 21 Biosafety Manual Template for BSL-2_[HOST] This is a general template for laboratory safety procedures. Biosafety level for ATCC cultures How does ATCC assign biosafety levels for cell lines? Purpose. is in line with the internal responsibility system promoted by the Act.

The manual provides a core set of biosafety UTA practices and procedures for the safe handling of known biohazards and potentially infectious materials. The UCD Centre for Research in Infectious Diseases (CRID) is located in a dedicated research building in UCD. When cell cultures are known to contain an infectious agent or an oncogenic virus, the cell line can be classified at the same level as that recommended for the agent. The UCD National Virus Reference Laboratory (NVRL) provides a diagnostic and reference service for clinicians investigating viral infections throughout Ireland. .

The Biological Safety Manual (or Biosafety Manual) has been adopted by Arizona State University to be a resource for information, guidelines, policies and procedures that will crid biosafety manual for bl2 cell lines ucd dublin enable safe research and to help eliminate, or reduce, the potential for exposure to biohazards. Box Seattle, WA crid biosafety manual for bl2 cell lines ucd dublin Phone: Institutional Biosafety Committee Bldg , Rm N Campus Box F Aurora, CO Office: / Fax: Email: IBC@[HOST] FAQ's. ) Designation: Human embryonic kidney cell line, permanent cell line 2.

These policies and procedures are designed to safeguard personnel and the environment from biologically hazardous materials and to comply with federal, state, and local regulatory crid biosafety manual for bl2 cell lines ucd dublin requirements. Cell lines are often immortalised by artificial expression of proteins which specifically cause the knockout of important cancer suppression genes crid biosafety manual for bl2 cell lines ucd dublin or the activation of proto-oncogenes. University Environmental Health and Safety. Risk Group 1, or unknown, or potentially infectious agents (including cell lines) 2. materials addressed in this manual and of interest to the Institutional Biosafety Committee (IBC) include the following: 1. WHO Laboratory Biosafety Manual crid biosafety manual for bl2 cell lines ucd dublin Having served for global biosafety community – Hereinafter, “WHO Manual” – “ For more than 20 30 years, since it was first published in , the Laboratory Biosafety Manual has provided practical guidance on biosafety techniques for use in laboratories at all levels.

The Princeton University Biosafety Manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the crid biosafety manual for bl2 cell lines ucd dublin potential for exposure to biological hazards. Mar 19,  · Choosing a Backup Generator Plus 3 LEGAL House Connection Options - Transfer Switch and More - Duration: Bailey Line Road Recommended for you. Uncontaminated Cell Cultures High Cell lines with endogenous biological agents or T-cells infected with HIV would require Biosafety Level 3 Primary cells from blood or lymphoid cells of human or simian origin Containment level appropriate to the risk NB: Any work that could give rise to.

These policies and procedures are designed to safeguard personnel and the environment from biologically hazardous materials and to comply with federal, state. This manual shall be adopted directly by Principal Investigators (PI) for use in their teaching / research laboratories, unless the PI generates their own biosafety manual. Biosafety Manual for Molecular Oncology Laboratory 3 precautions to prevent exposures, and the exposure evaluation procedures. The manual is part of MSU's biosafety program, which was established to accomplish the following goals. Biosafety Manual IBC-approved version (May 18, ) Printed copies are not official versions of this manual.

The Institutional Biosafety Committee (IBC) shall: • Review rDNA research conducted at or sponsored by the University for compliance with the. University of Washington. trainings required by the University, the University Biosafety Manual, the NIH Guidelines, the Occupational Safety and Health Administration (OSHA) Bloodborne Pathogen Standard (human-derived materials, including human cell lines: codified in 29 CFR ) and other local, state and federal regulations that apply to research.

crid biosafety manual for bl2 cell lines ucd dublin The purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at Boston University. This biosafety manual has been developed by the Environmental Health crid biosafety manual for bl2 cell lines ucd dublin and Safety (EHS) Department at Michigan State University. For questions please contact the Biosafety Program at biosafety@[HOST] or Emergency Contact X. Oct 16,  · Biosafety Levels (BSL) Research and teaching activities involving infectious agents requires prior approval by the UCSD Institutional Biosafety Committee (IBC) via the Biohazard Use Authorization (BUA) review process. D Worker Qualifications.

Refer to the Occupational Medicine Guidelines for more information. The manual sets forth a Biosafety Protocol, which provides a complete program of administration controls, medical surveillance, vaccination and crid biosafety manual for bl2 cell lines ucd dublin containment strategies for reducing risk posed crid biosafety manual for bl2 cell lines ucd dublin by biohazardous agents. Providing support, in the form of risk assessment, review, consultation and training, for research at UC Berkeley that involves the use of recombinant DNA, biohazardous materials and biological toxins. University Environmental Health and Safety for the respective campus will be responsible for: Preparing the Biosafety Manual and reviewing as needed or at least annually;.

This manual (Biosafety Protocol) has been developed as part of Stevens Institute of Technology’s Biosafety program. The Institutional Biosafety Committee (IBC) shall: • Review rDNA research conducted at or sponsored by the University for compliance with the NIH Guidelines, and approve those research projects that are crid biosafety manual for bl2 cell lines ucd dublin found to conform with the NIH Guidelines;. This Biosafety Manual applies to all University of Notre Dame lab personnel handling and/or potentially exposed to biological hazards in research and teaching laboratories. Biological agents listed by National Institutes of Health in Risk Group 2, 3 & 4. University Environmental Health and Safety. This training must be completed annually.

Anyone working in a BSL2 laboratory must follow - the procedures and policies of this Biosafety Manual. Ask Question Asked 4 years, 3 months ago. This is specifically designed for research on the pathogenesis of a range of infectious diseases. Biosafety Manual Revised March Page 1 of Institutional BIOSAFETY MANUAL The purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at Boston University and Boston Medical Center. ” – WHO web site The 3..

Each laboratory is required to have a lab-specific biosafety manual and cannot be borrowed or copied from another lab. The UCD National Virus Reference Laboratory (NVRL) provides a crid biosafety manual for bl2 cell lines ucd dublin diagnostic and reference service for clinicians investigating viral infections throughout Ireland. This is the third, revised edition of the booklet ‘Biosafety in the laboratory’. Three broad.

Providing support, in the form of risk assessment, review, consultation and training, for research at UC Berkeley that involves the use of recombinant DNA, biohazardous materials and biological toxins. Biosafety Manual for Molecular Oncology Laboratory 2 The Molecular Oncology Laboratory is a BSL-2 facility. Biosafety Level 4 (BSL4): dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease. The General Laboratory Safety training session is a campus requirement for all incoming faculty, staff, and students who will work in laboratory space. Purpose. Biosafety in the laboratory 3rd, revised edition. WHO Laboratory Biosafety Manual Having served for global biosafety community – Hereinafter, “WHO Manual” – “ For more than 20 30 years, since it was first published in , the Laboratory Biosafety Manual has provided practical guidance on biosafety techniques for use in laboratories crid biosafety manual for bl2 cell lines ucd dublin at all levels. Recombinant and Synthetic nucleic acids, Transgenic animals and plants, Human Gene Transfer, Pathogenic agents, Bloodborne and Aerosol Transmissible pathogens, Select agents and Toxins.

materials addressed in this manual and of interest to the Institutional Biosafety Committee (IBC) include the following: 1. Institutional Biosafety Manual, Version crid biosafety manual for bl2 cell lines ucd dublin Page 6 of This biosafety manual has been developed by the Environmental Health and Safety (EHS) Department at Michigan State University. Biosafety Manual Template for PI's of BSL-2 Laboratories 21 Biosafety Manual Template for BSL-2_[HOST] This is a general template for laboratory safety procedures. Standard Microbiological Practices: 1. The purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at Boston University.

The Department of Environmental Health. WHO Laboratory Biosafety Manual Having served for global biosafety community – Hereinafter, “WHO Manual” – “ For more than 20 30 years, since it was first published in , the Laboratory Biosafety Manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. In , the International Society for Advancement of Cytometry produced a guideline for cell-sorting core facilities to assist with understanding risks and biosafety terms. At the time of inspection, applicable documents listed below crid biosafety manual for bl2 cell lines ucd dublin will be reviewed for completion. This manual shall be adopted directly by Principal crid biosafety manual for bl2 cell lines ucd dublin Investigators (PI) for use in their teaching / research laboratories, unless the PI generates their own biosafety manual.

This program has. Personnel receive annual updates or additional training as necessary for procedural or policy changes. BIOSAFETY MANUAL. crid biosafety manual for bl2 cell lines ucd dublin The table below contains a . rd. Each laboratory space where biohazardous materials are used is assigned one of 3 internationally recognized biosafety crid biosafety manual for bl2 cell lines ucd dublin levels or BSL.

Biosafety Procedures, guidelines and resources to support safe laboratory and clinical research crid biosafety manual for bl2 cell lines ucd dublin involving potentially hazardous biological agents crid biosafety manual for bl2 cell lines ucd dublin and materials. Refer to the Occupational Medicine Guidelines for more information. The laboratory is affiliated to the University College Dublin School of Medicine. The following standard and special practices apply to agents assigned to Biosafety Level 2: A.

The Biological Safety Manual (or Biosafety Manual) has been adopted by Arizona State University to be a resource for information, guidelines, policies and procedures that will enable safe research and to help eliminate, or reduce, the potential for exposure to biohazards. Anyone working in a BSL2 laboratory must follow - the procedures and policies of this Biosafety Manual. ” – WHO web site The 3 rd. UCIMC School of Medicine clinical locations are not covered by this manual. Recombinant DNA 3. 5 D Biosafety Cabinets and Airborne Contamination D Manipulation Techniques for Minimizing Aerosols. The descriptions of biosafety levels (BSL) 1 – 4 parallel those in the NIH Guidelines for research involving recombinant DNA. BIOSAFETY MANUAL.

EHS PI Responsibilities – This course is required for all principal investigators conducting or overseeing research covered by a BUA. Workplace exposure to human blood, tissues, cell lines, and other potentially infectious materials (OPIM), as defined by the OSHA Bloodborne Pathogen Standard (29 CFR), requires medical surveillance and annual Bloodborne Pathogen Exposure Control Training. These policies and procedures are designed to. WHO Laboratory Biosafety Manual Having served for global biosafety community – Hereinafter, “WHO Manual” – “ For more than 20 30 years, since it was first published in , the Laboratory Biosafety Manual has provided practical guidance on crid biosafety manual for bl2 cell lines ucd dublin biosafety techniques for use in laboratories at all levels. This training must be completed. For questions please contact the Biosafety Program at biosafety@[HOST] or Emergency Contact X.

University College Dublin Biosafety Manual Rev. (PI) but is shared with the Institutional Biosafety Committee (IBC), biosafety professionals, laboratory staff and other stakeholders which may include lab animal veterinarians and caretakers, farm staff, operations staff, occupational health practitioners, etc.A. The UCI Biosafety Manual applies to all UCI faculty, staff, hosted visitors, students, participating guests and volunteers, contract laborers, supplemental personnel and employees of firms working at locations where UCI has management control of specific biohazards. Regulatory Requirements and Guidelines Guidelines developed by the National Institutes of Health (NIH) and the Centers for Disease Control and Prevention (CDC) form the basis for the biosafety practices included in this manual. The NVRL have provided a virology diagnostic service to the Irish health service for over forty years.

Risk Group 1, or unknown, or potentially infectious agents (including cell lines) 2. 3rd revised edition, May The biosafety of laboratory work is the central focal point of this booklet. University Environmental Health and Safety for the respective campus will be responsible for: Preparing the Biosafety Manual and . a) Risk Groups In many countries, infectious agents are categorized into risk groups based on their relative risk.

The UCI Biosafety Manual applies to all UCI faculty, staff, hosted visitors, students, participating guests and volunteers, contract laborers, supplemental personnel and employees of firms working at locations where UCI has management control of specific biohazards. Material Safety Data Sheet crid biosafety manual for bl2 cell lines ucd dublin for Cell Cultures (Biosafety Level 1) 1. Jan 24, crid biosafety manual for bl2 cell lines ucd dublin  · Human or non-human primate tissue or fluids, cell lines transformed with mammalian viruses, and human cell lines I. Before using the printed copy, verify that it is the most current version. ” – WHO web site The 3. The University of Texas at Arlington (UTA) Biosafety Manual has been prepared by the Environmental Health and Safety Office (EH&S) as a guideline for biological research at. Any proposed changes, e.

The McGill University Biosafety Manual is designed as an informational guide crid biosafety manual for bl2 cell lines ucd dublin to those who handle or work in close proximity with potentially infectious materials. The Princeton University Biosafety Manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. This biosafety manual must be available and accessible to all crid biosafety manual for bl2 cell lines ucd dublin laboratory personnel. personnel, biohazardous agent, location. ATCC assigns biosafety level (BSL) designations for the purposes of safe shipment, according to whether or not at the time of accessioning it is known that the cell line is harboring a known virus or any portion of a virus which causes human disease. The hazards presented by radiation are of physical rather than biological origin and thus are not covered in the Biosafety Manual;. The purpose of this Biosafety Manual is to define policies and procedures pertaining to the use of biological materials in research at Montana State University (MSU). This biosafety manual is applicable to all laboratory, research, service, and support activities that may involve exposure to bio-hazardous agents or materials and research that comes under the purview of the Radford.

4. Workplace exposure to human blood, tissues, cell lines, and other potentially infectious materials (OPIM), as defined by the OSHA Bloodborne Pathogen Standard (29 CFR), requires medical surveillance and annual Bloodborne Pathogen Exposure Control Training. Institutional Biosafety Committee Bldg , Rm N Campus Box F Aurora, CO Office: / Microsoft Word - UCD IBC Roster The Biosafety Manual is designed in accordance with NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules and the Biosafety in Microbiological and Biomedical Laboratories (BMBL, 5 th Edition) to assure laboratory personnel that – with proper precautions. SEPTEMBER Environmental Health and Safety Department.g.

Laboratory biosafety is the process of applying a combination of administrative controls, containment principles, practices and procedures, crid biosafety manual for bl2 cell lines ucd dublin safety equipment, emergency preparedness, and facilities to enable laboratory staff to work safely with potentially infectious microorganisms; biosafety also aims at preventing unintentional exposure to pathogens or their accidental release. Recombinant DNA 3. Cell line immortalisation biosafety.

Biosafety Level 3 (BSL3): indigenous or exotic agents that may cause serious or potentially lethal disease. cell lines, non-human primate cell lines, human primary tissue, blood, or any other potentially infectious material. Use of human cell lines The use of human cell lines and tissues in the laboratory presents potential hazards. Only a few alterations have been made, especially in the text concerning decontamination and inacti-vation. This Biosafety Manual applies to all University of Notre Dame lab personnel handling and/or potentially exposed to biological hazards in research and teaching laboratories.

Handling cell cultures and human tissues. This Laboratory Biosafety Manual • cell lines and other tissue cultures • nucleic acids, such as DNA derived from. crid biosafety manual for bl2 cell lines ucd dublin Prior to taking Understanding Biosafety, you should have already completed the online crid biosafety manual for bl2 cell lines ucd dublin training session “General Laboratory Safety”. Institutional Biosafety Manual, Version Page 6 of (PI) but is shared with the Institutional Biosafety Committee (IBC), biosafety professionals, laboratory staff and other stakeholders which may include lab animal veterinarians and caretakers, farm staff, operations staff, occupational health practitioners, etc. Other primate. Biosafety Manual Revised March Page 8 of • Biosafety Officer The Biosafety Officer (BSO) is responsible for provi ding guidance on the safe handling of biological agents and overall management of the Biosafety Program. Biosafety Procedures, guidelines and resources to crid biosafety manual for bl2 cell lines ucd dublin support safe laboratory and clinical research involving potentially hazardous biological agents and materials.

1 2 Issued January Introduction The Safety Health and Welfare At Work (Biological Agents) Regulations (SI ) defines a biological agent as ‘ a micro-organism including those which have been genetically modified, a cell culture and a human endoparasite, which may be. The BSO is an ex-officio member of the IBC.A. Biosafety Level 2 (BSL2): agents of moderate potential hazard to personnel and the environment.

Biosafety level for ATCC cultures How does ATCC assign biosafety levels for cell lines? FAQ Home. Company Identification CLS Cell Lines Service GmbH Dr. Biosafety Manual. ” – WHO web site The 3 rd. Find Answers.

The NVRL have provided a virology diagnostic service to the Irish health service for over forty years. Information is . Guidance on Working with Cell Cultures Guidance 1. INSTITUTIONAL BIOSAFETY Indian regulations require that every organisation intending to conduct research on genetically modified organisms (GMOs) should constitute an Institutional Biosafety Committee (IBSC) to approve and periodically review the biosafety aspects of the research projects. Box Seattle, WA Phone: Manual. These potential hazards include bloodborne pathogens such as HBV, HIV, HCV, HTLV, EBV, HPV and CMV, as well as agents such as Mycobacterium tuberculosis that may be present in human lung tissue. If exposure to human pathogens, blood, tissue, cell lines, or OPIM occurs or is suspected to have occurred while at work. As this manual does not address the chemical and physical hazards commonly encountered in the lab, it is to be regarded as an addendum to the Laboratory Safety Manual.

ATCC assigns biosafety level (BSL) designations for the purposes of safe shipment, according to whether or not at the time of accessioning it is known that the cell line is harboring a known virus or any portion of a virus. Use of human cell lines The use of human cell lines and tissues in the laboratory presents potential hazards. The laboratory is affiliated to the University College Dublin School of Medicine. The manual provides a core set of biosafety UTA practices and procedures for the safe handling of known biohazards and potentially infectious materials.

Regulatory crid biosafety manual for bl2 cell lines ucd dublin Requirements and Guidelines Guidelines developed by the crid biosafety manual for bl2 cell lines ucd dublin National Institutes of Health (NIH) and the Centers for Disease Control and Prevention (CDC) form the basis for the biosafety practices included in this manual. Work with animals/animal tissues or vectors known or suspected to be reservoirs of RG2 crid biosafety manual for bl2 cell lines ucd dublin or RG3 infectious agents when such work increases potential exposure risks to personnel or other animals. The BSL categories are divided up by risk of disease combined with availability of preventive and therapeutic treatments.

The General Laboratory Safety training session is a campus requirement for all incoming faculty, staff, and students who will work in laboratory space. such as unfixed human tissues, primary human cell lines, and certain body fluids. The Department of . Recombinant and Synthetic nucleic acids, Transgenic animals and plants, crid biosafety manual for bl2 cell lines ucd dublin Human Gene Transfer, Pathogenic agents, Bloodborne and Aerosol Transmissible pathogens, Select agents and Toxins. Prior to taking Understanding Biosafety, you should have already completed the online training session “General Laboratory Safety”. Access crid biosafety manual for bl2 cell lines ucd dublin to the laboratory is limited or restricted at the discretion of the laboratory director. UCIMC School of Medicine clinical locations are not covered by this manual.

Three broad. When cell cultures are known to contain an infectious agent or an oncogenic virus, the cell line can be classified at . This biosafety manual must be available and accessible to all laboratory crid biosafety manual for bl2 cell lines ucd dublin personnel. Each laboratory is required to have a lab-specific biosafety manual and cannot be borrowed or copied from another lab. At the time of inspection, applicable documents listed below will be reviewed for completion. 14 Biosafety Manual Familiarity with exposure routes, symptoms, and treatment methods will provide better preparation in the event of exposure to the human pathogens, blood, tissues, cell lines, or OPIM.

Jan 24, · Human or non-human primate tissue or fluids, cell lines transformed with mammalian viruses, and human cell lines I. 14 Biosafety Manual Familiarity with exposure routes, symptoms, and treatment methods will provide better preparation in the event of exposure to the human pathogens, blood, tissues, cell lines, or OPIM. As such, the Concordia Biosafety manual is based on these guidelines as well as good laboratory practices. 8 D Eppelheim Germany Emergency phone number: +49 (0) BIOSAFETY CERTIFICATE To meet the requirements of The University of Toronto Biosafety Program, a Biosafety Certification Form must crid biosafety manual for bl2 cell lines ucd dublin be approved by and filed with the Biosafety office prior to purchasing, importing or conducting projects involving biohazards. This Laboratory Biosafety Manual addresses the safety requirements for working with biological materials as defined below. This biosafety manual is applicable to all laboratory, research, service, and support activities that may involve exposure to bio-hazardous agents or materials and research that comes under the purview of the Radford. University College Dublin Biosafety Manual Rev. Product Identification Name of cell line: HEK (CLS order no.

accessed at RU EHS programs. In this booklet you will find all the information that was already present in the earlier editions. The Biosafety Manual is designed in accordance with NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules and the Biosafety in Microbiological and Biomedical Laboratories (BMBL, 5 th Edition) to assure laboratory personnel that – with proper precautions. accessed at RU EHS programs. Handling cell cultures and human tissues. These potential hazards include bloodborne pathogens such as HBV, HIV, HCV, HTLV, EBV, HPV and CMV, as well as then the Office of Biosafety will review it .

Human and non-human primate cells including cell lines, tissue, blood and potentially infectious fluids. SEPTEMBER Environmental Health and Safety Department. 4. Eckener-Str. Dr Suzie Coughlan Principal Clinical Scientist National Virus Reference Laboratory Dr Cillian F De Gascun crid biosafety manual for bl2 cell lines ucd dublin Director, National Virus Reference Laboratory National Virus Reference Laboratory Dr Jaythoon Hassan Senior Clinical Scientist National Virus Reference Laboratory The McGill University Biosafety Manual is designed as an informational guide to those who handle or work in close proximity with potentially infectious materials. These policies and procedures are designed to safeguard MSU Human and non-human primate cells including cell lines, tissue, blood and potentially infectious fluids. The University of Texas at Arlington (UTA) Biosafety Manual has been prepared by the Environmental Health and Safety Office (EH&S) as a guideline for biological research at.

such as unfixed human tissues, primary human cell lines, and certain body fluids. The manual is part of MSU's biosafety program, which was established to accomplish the following goals. Prerequisite. Biosafety Level 1 (BSL1): well-characterized agents not consistently crid biosafety manual for bl2 cell lines ucd dublin known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.

Biological agents listed by National Institutes of Health in Risk Group 2, 3 & 4. 1 2 Issued January Introduction The Safety Health and Welfare At Work (Biological Agents) Regulations (SI ) defines a biological crid biosafety manual for bl2 cell lines ucd dublin agent as ‘ a micro-organism including those which have been genetically modified, a cell culture and a human endoparasite, which may be. This centre has Biosafety level 2 (BL2) and BL3 (+) containment facilities and dedicated.


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